BLOOD SYSTEM CONDITION IN RATS WITH ALLOXAN-INDUCED DIABETES MELLITUS AND ITS CORRECTION WITH 2-MORPHOLINO-5-PHENYL-6H-1,3,4-THIADIAZINE HYDROBROMIDE
DOI: 10.22138/2500-0918-2026-23-2-216-231
I.A. Shadrin¹, V.V. Emelianov¹, I.F. Gette², K.V. Sokolova¹,², S.L.
Khatsko¹, T.A. Tseitler¹, L.P. Sidorova¹, N.N. Mochulskaya¹,
I.G. Danilova¹,², V.A.Chereshnev¹,²
¹Ural Federal University named after the first President of Russia B.N. Yeltsin, Yekaterinburg, Russian Federation.
²Institute of Immunology and Physiology
of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russian Federation.
Abstract. The aim of the study was to evaluate the state of peripheral blood and bone marrow hematopoiesis in rats modeling alloxan-induced diabetes mellitus and its correction with compound L-17 from the 2,5-substituted 6H-1,3,4-thiadiazine series. Materials and methods: the study was conducted on 20 male Wistar rats. Peripheral blood was analyzed using a hematology analyzer. The state of bone marrow hematopoiesis was assessed by counting myelograms, as well as determining the total number of erythroblastic islets and their distribution by maturity. Results: the development of alloxan-induced diabetes mellitus causes complex changes in the blood system in rats, affecting both the cellular composition of the peripheral blood and bone marrow hematopoiesis. Compound L-17 is able to partially correct these changes. Its ability to activate erythropoiesis and inhibit lympho- and monocytopoiesis in the bone marrow is of key importance. Conclusions: the study revealed changes in the cellular composition of the peripheral blood and bone marrow of rats with alloxan-induced diabetes and its correction with 2-morpholino-5-phenyl-6H-1,3,4-thiadiazine hydrobromide.
Keywords: 2-morpholino-5-phenyl-6H-1,3,4-thiadiazine, hydrobromide, alloxan, diabetes mellitus, erythroblastic islands, bone marrow, hematopoiesis, peripheral blood
There is no conflict of interest.
Contact details of the corresponding author:
Ilya Alekseevich Shadrin
i.a.shadrin@urfu.ru
Received: 20.02.2026
For citation: Shadrin I.A., Emelianov V.V., Gette I.F., Sokolova K.V., Khatsko S.L., Tseitler T.A., Sidorova L.P., Mochulskaya N.N., Danilova I.G., Chereshnev V.A. Blood system condition in rats with alloxan-induced diabetes mellitus and its correction with 2-morpholino-5-phenyl-6h-1,3,4-thiadiazine hydrobromide [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2026, Vol. 23, no. 2, pp. 216–231. DOI: 10.22138/2500-0918-2026-23-2-216-231 (In Russ)
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Authors
Ilya A. Shadrin
Ural Federal University named after the first President of Russia B.N. Yeltsin
Senior Lecturer, Department of Immunochemistry
19 Mira St., Yekaterinburg, 620002, Russian Federation
i.a.shadrin@urfu.ru
Viktor V. Emelianov
Ural Federal University named after the first President of Russia B.N. Yeltsin
Ph.D., Associate Professor, Head of the Department of Medical Biochemistry and Biophysics, Associate Professor of the Department of Immunochemistry
19 Mira St., Yekaterinburg, 620002, Russian Federation
v.v.emelianov@urfu.ru
Irina F. Gette
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Ph.D., Senior Researcher, Laboratory of Morphology and Biochemistry
106 Pervomaiskaya St., Yekaterinburg, 620049, Russian Federation
i.goette@yandex.ru
Ksenia V. Sokolova
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Ph.D., Senior Researcher, Laboratory of Morphology and Biochemistry
106 Pervomaiskaya St., Yekaterinburg, 620049, Russian Federation
Ural Federal University named after the first President of Russia B.N. Yeltsin
Laboratory Assistant, Department of Medical Biochemistry and Biophysics
19 Mira St., Yekaterinburg, 620002, Russian Federation
xenia.socolova@gmail.com
Sergey L. Khatsko
Ural Federal University named after the first President of Russia B.N. Yeltsin
Head of Anatomical and Physiological Experimental Laboratory
19 Mira St., Yekaterinburg, 620002, Russian Federation
sergey.khatsko@urfu.ru
Tatyana A. Tseitler
Ural Federal University named after the first President of Russia B.N. Yeltsin
PhD, Associate professor of the Department of organic and biomolecular chemistry
19 Mira St., Yekaterinburg, 620002, Russian Federation
t.a.tceytler@urfu.ru
Larisa P. Sidorova
Ural Federal University named after the first President of Russia B.N. Yeltsin
PhD, Associate Professor
19 Mira St., Yekaterinburg, 620002, Russian Federation
vlapp@list.ru
Natalia N. Mochulskaya
Ural Federal University named after the first President of Russia B.N. Yeltsin
PhD in Chemistry, Associate Professor of the Department of Immunochemistry
19 Mira St., Yekaterinburg, 620002, Russian Federation
n.n.mochulskaia@urfu.ru
Irina G. Danilova
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Doctor of Biological Sciences, Associate Professor, Head of the Laboratory of Morphology and Biochemistry
106 Pervomaiskaya St., Yekaterinburg, 620049, Russian Federation
Ural Federal University named after the first President of Russia B.N. Yeltsin
Professor, Department of Medical Biochemistry and Biophysics
19 Mira St., Yekaterinburg, 620002, Russian Federation
ig-danilova@yandex.ru
Valery A. Chereshnev
Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences
Academician of the Russian Academy of Sciences, Doctor of Medical Sciences, Professor, Scientific Director of the Institute
106 Pervomaiskaya St., Yekaterinburg, 620049, Russian Federation
Ural Federal University named after the First President of Russia B.N. Yeltsin
Head of the Department of Immunochemistry
19 Mira St., Yekaterinburg, 620002, Russian Federation
v.chereshnev@mail.ru